Effects of Advanced Glycation End Products on Differentiation and Function of Osteoblasts and Osteoclasts.

ABSTRACT:

Background

Risk of fragility fractures increases in patients with diabetes mellitus, independent of bone mineral density. In the present study, the effects of advanced glycation end products (AGEs) on differentiation and function of osteoblasts and osteoclasts were investigated.

Methods

AGEs and 25 mM glucose were administered to marrow-derived macrophages and MCT3T3-E1 cells. The effects of AGEs on osteoclast differentiation was investigated using tartrate-resistant acid phosphatase (TRAP) assay. The effects of AGEs on osteoblast differentiation was investigated using alkaline phosphatase (ALP) activity and bone nodule formation assays. Expression of osteoclast-specific and osteoblast-specific genes and effects on cell signaling pathways associated with cell differentiation

SUBMITTER: Park SY 

PROVIDER: S-EPMC8476938 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature