X-Ray Causes mRNA Transcripts Change to Enhance Orai2-Mediated Ca²⁺ Influx in Rat Brain Microvascular Endothelial Cells.

ABSTRACT: Background: Radiation-induced brain injury is a serious and treatment-limiting complication of brain radiation therapy. Although endothelial cell dysfunction plays a critical role in the development of this pathogenesis, the underlying molecular mechanisms remain elusive. Methods: Primary cultured rat brain microvascular endothelial cells (BMECs) were divided into five groups without or with exposure of x-rays delivered at 5 Gy or 20 Gy. For the irradiated groups, cells were continued to cultivate for 12 or 24 h after being irradiated. Then the mRNA libraries of each group were established and applied for next-generation sequencing. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were conducted to analyze the sequencing results. Quantitat

SUBMITTER: Xu F 

PROVIDER: S-EPMC8477418 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature