Isolation and characterization of urine microvesicles from prostate cancer patients: different approaches, different visions.

ABSTRACT:

Background

Because of their specific and biologically relevant cargo, urine extracellular vesicles (EVs) constitute a valuable source of potential non-invasive biomarkers that could support the clinical decision-making to improve the management of prostate cancer (PCa) patients. Different EV isolation methods differ in terms of complexity and yield, conditioning, as consequence, the analytical result.

Methods

The aim of this study was to compare three different isolation methods for urine EVs: ultracentrifugation (UC), size exclusion chromatography (SEC), and a commercial kit (Exolute® Urine Kit). Urine samples were collected from 6 PCa patients and 4 healthy donors. After filtered through 0.22 µm filters, urine was divided in 3 equal volumes to perform EVs isolation with ea

SUBMITTER: Garcia-Flores M 

PROVIDER: S-EPMC8477576 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature