Odontogenic gene expression profile of human dental pulp-derived cells under high glucose influence: a microarray analysis.

ABSTRACT: Hyperglycemia, a major characteristic of diabetes, is considered to play a vital role in diabetic complications. High glucose levels have been found to inhibit the mineralization of dental pulp cells. However, gene expression associated with this phenomenon has not yet been reported. This is important for future dental therapeutic application.

Objective

Our study aimed to investigate the effect of high glucose levels on mineralization of human dental pulp-derived cells (hDPCs) and identify the genes involved.

Methodology

hDPCs were cultured in mineralizing medium containing 25 or 5.5 mM D-glucose. On days 1 and 14, RNA was extracted and expression microarray performed. Then, differentially expressed genes (DEGs) were selected for further validation using the reverse transcrip

SUBMITTER: Horsophonphong S 

PROVIDER: S-EPMC8477757 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature