Evaluating Endosomal Escape of Caspase-3-Containing Nanomaterials Using Split GFP.

ABSTRACT: The ability for biologics to access intracellular targets hinges on the translocation of active, unmodified proteins. This is often achieved using nanoscale formulations, which enter cells through endocytosis. This uptake mechanism often limits the therapeutic potential of the biologics, as the propensity of the nanocarrier to escape the endosome becomes the key determinant. To appropriately evaluate and compare competing delivery systems of disparate compositions, it is therefore critical to assess endosomal escape efficiencies. Unfortunately, quantitative tools to assess endosomal escape are lacking, and standard approaches often lead to an erroneous interpretation of cytosolic localization. In this study we use a split-complementation endosomal escape (SEE) assay to evaluate levels of c

SUBMITTER: Anson F 

PROVIDER: S-EPMC8477791 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature