Dataset Information

The association between single nucleotide polymorphism in vitamin D receptor and calcium oxalate urolithiasis in dogs.

ABSTRACT:

Background

Polymorphisms of the vitamin D receptor (VDR) are associated with calcium oxalate (CaOx) nephrolithiasis in humans.

Objectives

To investigate the association between VDR polymorphisms and susceptibility to CaOx urolithiasis in dogs.

Animals

Thirty-five dogs with CaOx urolithiasis were compared with 40 stone-free dogs.

Methods

This was a case-control study. Two VDR gene polymorphisms (rs851998024 and rs852900542) were detected by specific TaqMan real-time polymerase chain reaction assay, and their relationship with serum 1,25-dihydroxyvitamin D, serum and urinary electrolyte concentrations was evaluated.

Results

The distribution of the rs852900542 polymorphism was significantly different between the case and the control dogs (x² = 6.369, P = .04). Dogs with a CC or CT genotype had an increased risk of CaOx stones than those with the TT genotype (odds ratio = 3.82, 95% confidence interval 1.04-13.98). The CaOx dogs with the TT genotype had a significantly lower urinary calcium-to-creatinine ratio than the CT+CC genotypes. 1,25-(OH)2D concentrations did not differ between the cases and the controls (308.7 ± 217.4 vs 286.7 ± 185.1 pg/mL, P = .45).

Conclusions and clinical importance

This finding suggests that vitamin D metabolism might play a role in CaOx stone formation in dogs.

SUBMITTER: Chamsuwan S

PROVIDER: S-EPMC8478019 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature

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Publications

The association between single nucleotide polymorphism in vitamin D receptor and calcium oxalate urolithiasis in dogs.

Chamsuwan Sumonwan S Angkanaporn Kris K Dissayabutra Thasinas T Chuaypen Natthaya N Buranakarl Chollada C

Journal of veterinary internal medicine 20210728 5

<h4>Background</h4>Polymorphisms of the vitamin D receptor (VDR) are associated with calcium oxalate (CaOx) nephrolithiasis in humans.<h4>Objectives</h4>To investigate the association between VDR polymorphisms and susceptibility to CaOx urolithiasis in dogs.<h4>Animals</h4>Thirty-five dogs with CaOx urolithiasis were compared with 40 stone-free dogs.<h4>Methods</h4>This was a case-control study. Two VDR gene polymorphisms (rs851998024 and rs852900542) were detected by specific TaqMan real-time p ...[more]

PMID: 34322901

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Project description:BackgroundThere is an ongoing controversy regarding the expression of vitamin D receptor (VDR) and binding protein (VDBP) genes, as well as their polymorphisms, in periodontitis. We examined eight single nucleotide polymorphisms (SNPs) and performed a transcriptome-level bioinformatics analysis to clarify their relationship with periodontitis.MethodsTo explore VDR and VDBP polymorphisms, 600 subjects were included, including 307 patients with chronic periodontitis (CP) and 293 healthy controls. Genomic DNA was extracted from peripheral venous blood collected from each subject. A MassARRAY system was used to detect SNPs, including rs1544410G/A (BsmI), rs2228570C/T (FokI), rs7975232G/T (ApaI), rs731236T/C (TaqI), rs739837G/T, rs9729G/T, and rs3847987C/A in the VDR gene, and rs7041A/C in the VDBP gene. Then, we analyzed transcriptome sequencing datas of gingival tissues from two single-cell transcriptome sequencing studies to identify differential expression profiles. The objective was to further explore the potential association between VDR gene and gingival tissues in individuals with CP.ResultsThe regression analysis model revealed a significant relationship between rs739837G/T (P = 0.04) and rs7041A/C (P = 0.03) polymorphisms and CP susceptibility. Subjects carrying the TT genotype of rs739837 showed a decreased risk of developing CP compared to those carrying the GG + GT genotype (OR = 0.53, 95% CI = 0.29-0.99). Individuals carrying the AC + CC genotype of rs7041 showed a reduced risk of developing CP compared to those with the AA genotype (OR = 0.70, 95% CI = 0.51-0.97). Furthermore, allele C of rs7041 was found to have a protective effect against periodontitis (P = 0.03, OR = 0.75, CI = 0.58-0.98). However, no association was found between CP susceptibility and six other 6 SNPs (rs1544410, rs2228570, rs7975232, rs9729, rs731236, and rs3847987). Differential levels of VDR transcription were observed in gingival tissues during CP.ConclusionsVDR genetic variability and transcriptional expression are significant factors affecting susceptibility to CP. These findings suggested that rs739837 TT in VDR and rs7041 A/C in VDBP may be protective against periodontitis.

Dataset Information

The association between single nucleotide polymorphism in vitamin D receptor and calcium oxalate urolithiasis in dogs.

Background

Objectives

Animals

Methods

Results

Conclusions and clinical importance

Publications

The association between single nucleotide polymorphism in vitamin D receptor and calcium oxalate urolithiasis in dogs.

Similar Datasets

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