A Yeast Chronological Lifespan Assay to Assess Activity of Proteasome Stimulators.

ABSTRACT: Aging is characterized by changes in several cellular processes, including dysregulation of proteostasis. Current research has shown long-lived rodents display elevated proteasome activity throughout life and proteasome dysfunction is linked to shorter lifespans in a transgenic mouse model. The ubiquitin proteasome system (UPS) is one of the main pathways leading to cellular protein clearance and quality maintenance. Reduction in proteasome activity is associated with aging and its related pathologies. Small molecule stimulators of the proteasome have been proposed to help alleviate cellular stress related to unwanted protein accumulation. Here we have described the development of techniques to monitor the impact of proteasome stimulation in wild-type yeast and a strain that has impaired p

SUBMITTER: Maresh ME 

PROVIDER: S-EPMC8478123 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature