Ontology highlight
ABSTRACT:
SUBMITTER: Magnusson JP
PROVIDER: S-EPMC8478413 | biostudies-literature | 2021 Sep
REPOSITORIES: biostudies-literature
Magnusson Jens P JP Rios Antonio Ray AR Wu Lingling L Qi Lei S LS
eLife 20210909
The type V-A Cas12a protein can process its CRISPR array, a feature useful for multiplexed gene editing and regulation. However, CRISPR arrays often exhibit unpredictable performance due to interference between multiple guide RNA (gRNAs). Here, we report that Cas12a array performance is hypersensitive to the GC content of gRNA spacers, as high-GC spacers can impair activity of the downstream gRNA. We analyze naturally occurring CRISPR arrays and observe that natural repeats always contain an AT- ...[more]