SCIGA: Software for large-scale, single-cell immunoglobulin repertoire analysis.

ABSTRACT:

Background

B-cell immunoglobulin repertoires with paired heavy and light chain can be determined by means of 10X single-cell V(D)J sequencing. Precise and quick analysis of 10X single-cell immunoglobulin repertoires remains a challenge owing to the high diversity of immunoglobulin repertoires and a lack of specialized software that can analyze such diverse data.

Findings

In this study, specialized software for 10X single-cell immunoglobulin repertoire analysis was developed. SCIGA (Single-Cell Immunoglobulin Repertoire Analysis) is an easy-to-use pipeline that performs read trimming, immunoglobulin sequence assembly and annotation, heavy and light chain pairing, statistical analysis, visualization, and multiple sample integration analysis, which is all achieved by using a 1-

SUBMITTER: Ye H 

PROVIDER: S-EPMC8478610 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature