Live cell molecular analysis of primary prostate cancer organoids identifies persistent androgen receptor signaling.

ABSTRACT: Prostate Cancer (PC) is a disease with remarkable tumor heterogeneity that often manifests in significant intra-patient variability with regards to clinical outcomes and treatment response. Commonly available PC cell lines do not accurately reflect the complexity of this disease and there is critical need for development of new models to recapitulate the intricate hierarchy of tumor pathogenesis. In current study, we established ex vivo primary patient-derived cancer organoid (PDCO) cultures from prostatectomy specimens of patients with locally advanced PC. We then performed a comprehensive multi-parameter characterization of the cellular composition utilizing a novel approach for live-cell staining and direct imaging in the integrated microfluidic Stacks device. Using orthogonal flow cyto

SUBMITTER: Heninger E 

PROVIDER: S-EPMC8478748 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature