Bioengineering potato plants to produce benzylglucosinolate for improved broad-spectrum pest and disease resistance.

ABSTRACT: In traditional, small-scale agriculture in the Andes, potatoes are frequently co-cultivated with the Andean edible tuber Tropaeolum tuberosum, commonly known as mashua, which is believed to exert a pest and disease protective role due to its content of the phenylalanine-derived benzylglucosinolate (BGLS). We bioengineered the production of BGLS in potato by consecutive generation of stable transgenic events with two polycistronic constructs encoding for expression of six BGLS biosynthetic genes from Arabidopsis thaliana. First, we integrated a polycistronic construct coding for the last three genes of the pathway (SUR1, UGT74B1 and SOT16) into potato driven by the cauliflower mosaic virus 35S promoter. After identifying the single-insertion transgenic event with the highest transgene expre

SUBMITTER: Gonzalez-Romero ME 

PROVIDER: S-EPMC8478770 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature