Elimination of vision-guided target attraction in Aedes aegypti using CRISPR.

ABSTRACT: Blood-feeding insects, such as the mosquito, Aedes (Ae.) aegypti, use multiple senses to seek out and bite humans.^1,2 Upon exposure to the odor of CO₂, the attention of female mosquitoes to potential targets is greatly increased. Female mosquitoes are attracted to high-contrast visual cues and use skin olfactory cues to assist them in homing in on targets several meters away.^3-9 Within close range, convective heat from skin and additional skin odors further assist the mosquitoes' evaluation as to whether the object of interest might be a host.^10,11 Here, using CRISPR-Cas9, we mutated the gene encoding Op1, which is the most abundant of the five rhodopsins expressed in the eyes of Ae. aegypti. Using cage

SUBMITTER: Zhan Y 

PROVIDER: S-EPMC8478898 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature