Supercoiling and looping promote DNA base accessibility and coordination among distant sites.

ABSTRACT: DNA in cells is supercoiled and constrained into loops and this supercoiling and looping influence every aspect of DNA activity. We show here that negative supercoiling transmits mechanical stress along the DNA backbone to disrupt base pairing at specific distant sites. Cooperativity among distant sites localizes certain sequences to superhelical apices. Base pair disruption allows sharp bending at superhelical apices, which facilitates DNA writhing to relieve torsional strain. The coupling of these processes may help prevent extensive denaturation associated with genomic instability. Our results provide a model for how DNA can form short loops, which are required for many essential processes, and how cells may use DNA loops to position nicks to facilitate repair. Furthermore, our results

SUBMITTER: Fogg JM 

PROVIDER: S-EPMC8478907 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature