The identification of grain size genes by RapMap reveals directional selection during rice domestication.

ABSTRACT: Cloning quantitative trait locus (QTL) is time consuming and laborious, which hinders the understanding of natural variation and genetic diversity. Here, we introduce RapMap, a method for rapid multi-QTL mapping by employing F₂ gradient populations (F₂GPs) constructed by minor-phenotypic-difference accessions. The co-segregation standard of the single-locus genetic models ensures simultaneous integration of a three-in-one framework in RapMap i.e. detecting a real QTL, confirming its effect, and obtaining its near-isogenic line-like line (NIL-LL). We demonstrate the feasibility of RapMap by cloning eight rice grain-size genes using 15 F₂GPs in three years. These genes explain a total of 75% of grain shape variation. Allele frequency analysis of these genes u

SUBMITTER: Zhang J 

PROVIDER: S-EPMC8478914 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature