Protein expression reveals a molecular sexual identity of avian primordial germ cells at pre-gonadal stages.

ABSTRACT: In poultry, in vitro propagated primordial germ cells (PGCs) represent an important tool for the cryopreservation of avian genetic resources. However, several studies have highlighted sexual differences exhibited by PGCs during in vitro propagation, which may compromise their reproductive capacities. To understand this phenomenon, we compared the proteome of pregonadal migratory male (ZZ) and female (ZW) chicken PGCs propagated in vitro by quantitative proteomic analysis using a GeLC-MS/MS strategy. Many proteins were found to be differentially abundant in chicken male and female PGCs indicating their early sexual identity. Many of the proteins more highly expressed in male PGCs were encoded by genes localised to the Z sex chromosome. This suggests that the known lack of dosage compensatio

SUBMITTER: Soler L 

PROVIDER: S-EPMC8478952 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature