Loop Diuretics Inhibit Ischemia-Induced Intracellular Ca²⁺ Overload in Neurons via the Inhibition of Voltage-Gated Ca²⁺ and Na⁺ Channels.

ABSTRACT: One consequence of ischemic stroke is disruption of intracellular ionic homeostasis. Intracellular overload of both Na⁺ and Ca²⁺ has been linked to neuronal death in this pathophysiological state. The etiology of ionic imbalances resulting from stroke-induced ischemia and acidosis includes the dysregulation of multiple plasma membrane transport proteins, such as increased activity of sodium-potassium-chloride cotransporter-1 (NKCC-1). Experiments using NKCC1 antagonists, bumetanide (BMN) and ethacrynic acid (EA), were carried out to determine if inhibition of this cotransporter affects Na⁺ and Ca²⁺ overload observed following in vitro ischemia-acidosis. Fluorometric Ca²⁺ and Na⁺ measurements were performed using cultu

SUBMITTER: Katnik C 

PROVIDER: S-EPMC8479115 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature