Two facile approaches based on association complex with erythrosine-B for nano-level analysis of duloxetine: application to content uniformity.

ABSTRACT: Duloxetine is an antidepressant that exhibits its action by preventing the reuptake of serotonin and norepinephrine by neurons. In this analytical study, we developed two facile, sensitive methods for duloxetine analysis. Both methods rely on the formation of binary association complex between erythrosine-B and duloxetine in an acidic medium using spectrofluorimetric and resonance Rayleigh scattering (RRS) techniques. Spectrofluorimetric method simply uses the quenching property of the formed complex on the native fluorescence of erythrosine-B at an emission wavelength of 557.2 nm (λ _ex = 528.6), while RRS is based on detecting the enhancement in the RRS signal at 357.2 nm. The proposed methods have been validated according to the International Conference on Harmonization

SUBMITTER: Derayea SM 

PROVIDER: S-EPMC8479336 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature