Dysregulation of cholesterol homeostasis in human lung cancer tissue and tumour-associated macrophages.

ABSTRACT:

Background

Based on reports on elevated cholesterol levels in cancer cells, strategies to lower cholesterol synthesis have been suggested as an antitumour strategy. However, cholesterol depletion has also been shown to induce tumour-promoting actions in tumour-associated macrophages (TAMs).

Methods

We performed lipidomic and transcriptomic analyses of human lung cancer material. To assess whether the TAM phenotype is shaped by secreted factors produced by tumour cells, primary human monocyte-derived macrophages were polarized towards a TAM-like phenotype using tumour cell-conditioned medium.

Findings

Lipidomic analysis of lung adenocarcinoma (n=29) and adjacent non-tumour tissues (n=22) revealed a significant accumulation of free cholesterol and cholesteryl esters wit

SUBMITTER: Hoppstadter J 

PROVIDER: S-EPMC8479395 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature