Gold nanoparticle based plasmonic sensing for the detection of SARS-CoV-2 nucleocapsid proteins.

ABSTRACT: An inexpensive virus detection scheme with high sensitivity and specificity is desirable for broad applications such as the COVID-19 virus. In this article, we introduce the localized surface plasmon resonance (LSPR) principle on the aggregation of antigen-coated gold nanoparticles (GNPs) to detect SARS-CoV-2 Nucleocapsid (N) proteins. Experiments show this technique can produce results observable by the naked eye in 5 min with a LOD (Limits of Detection) of 150 ng/ml for the N proteins. A comprehensive numerical model of the LSPR effect on the aggregation of GNPs has been developed to identify the key parameters in the reaction processes. The color-changing behaviors can be readily utilized to detect the existence of the virus while the quantitative concentration value is characterized wi

SUBMITTER: Behrouzi K 

PROVIDER: S-EPMC8479426 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature