Citrate Stabilizes Hydroxylapatite Precursors: Implications for Bone Mineralization.

ABSTRACT: Mineralization of hydroxylapatite (HAp), the main inorganic phase in bone, follows nonclassical crystallization routes involving metastable precursors and is strongly influenced by organic macromolecules. However, the effect of small organic molecules such as citrate on the formation of HAp is not well constrained. Using potentiometric titration experiments and titration calorimetry, in combination with a multianalytical approach, we show that citrate stabilizes prenucleation species as well as a liquid-like calcium phosphate precursor formed before any solid phase nucleates in the system. The stabilization of a liquid-like precursor phase could facilitate infiltration into the cavities of the collagen fibrils during bone mineralization, explaining the enhancement of collagen-mediated mine

SUBMITTER: Ruiz-Agudo E 

PROVIDER: S-EPMC8479724 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature