Versatile Lipases from the Candida rugosa-like Family: A Mechanistic Insight Using Computational Approaches.

ABSTRACT: Lipases are enzymes able to catalyze the hydrolysis or synthesis of triglycerides, depending on the reaction conditions, whereas sterol esterases show the same ability on sterol esters. Structurally, both kinds of enzymes display an α/β-hydrolase fold, with a substrate-binding pocket formed by a hydrophobic cavity covered by a mobile lid. However, it has been reported that some lipases from the Candida rugosa-like family display wide substrate specificity on both triglycerides and sterol esters. Among them, enzymes with different biotechnological applications, such as the lipase isoenzymes produced by C. rugosa and the sterol esterase from Ophiostoma piceae, have been exhaustively characterized and their crystal structures are available. Differences in substrate affini

SUBMITTER: Rodriguez-Salarichs J 

PROVIDER: S-EPMC8479805 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature