HyRAD-X Exome Capture Museomics Unravels Giant Ground Beetle Evolution.

ABSTRACT: Advances in phylogenomics contribute toward resolving long-standing evolutionary questions. Notwithstanding, genetic diversity contained within more than a billion biological specimens deposited in natural history museums remains recalcitrant to analysis owing to challenges posed by its intrinsically degraded nature. Yet that tantalizing resource could be critical in overcoming taxon sampling constraints hindering our ability to address major evolutionary questions. We addressed this impediment by developing phyloHyRAD, a new bioinformatic pipeline enabling locus recovery at a broad evolutionary scale from HyRAD-X exome capture of museum specimens of low DNA integrity using a benchtop RAD-derived exome-complexity-reduction probe set developed from high DNA integrity specimens. Our new pipe

SUBMITTER: Toussaint EFA 

PROVIDER: S-EPMC8480185 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature