Identification of pathogenic Leptospira species and serovars in New Zealand using metabarcoding.

ABSTRACT: Leptospirosis is a zoonotic disease of global importance. The breadth of Leptospira diversity associated with both human and animal disease poses major logistical challenges to the use of classical diagnostic techniques, and increasingly molecular diagnostic tools are used for their detection. In New Zealand, this has resulted in an increase in positive cases reported nationally that have not been attributed to the infecting serovar or genomospecies. In this study, we used data from all pathogenic Leptospira genomes to identify a partial region of the glmU gene as a suitable locus for the discrimination of the infecting species and serovars of New Zealand-endemic Leptospira. This method can be used in culture and culture-independent scenarios making it flexible for diagnostics in humans, a

SUBMITTER: Wilkinson DA 

PROVIDER: S-EPMC8480790 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature