Unknown

Dataset Information

0

NMR resonance assignments of the PR-10 allergens Act c 8 and Act d 8 from golden and green kiwifruit.


ABSTRACT: Kiwifruits have become one of the most common food sources triggering allergic reactions. In patients suffering from birch pollen related food allergy, reactions result from initial sensitization to the birch (Betula verrucosa) pollen allergen Bet v 1, followed by immunological cross-reactivity to structurally homologous proteins in kiwifruit. Clinical symptoms range from scratching and itching of the oral cavity to more severe immunological reactions such as rhino conjunctivitis. In this work we assigned backbone and side chain 1H, 13C and 15N chemical shifts of the 17 kDa PR-10 allergens Act c 8.0101 and Act d 8.0101 from golden (Actinidia chinesis) and green (Actinidia deliciosa) kiwifruit by solution NMR spectroscopy. The chemical shift data confirm the characteristic Bet v 1 fold for both proteins, consisting of a seven-stranded antiparallel β-sheet interrupted by two short α-helices, along with a long C-terminal α-helix. Our data provide the basis for determining the three-dimensional solution structures of these proteins and characterizing their immunological cross-reactivity on a structural basis.

SUBMITTER: Zeindl R 

PROVIDER: S-EPMC8481192 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

NMR resonance assignments of the PR-10 allergens Act c 8 and Act d 8 from golden and green kiwifruit.

Zeindl Ricarda R   Tollinger Martin M  

Biomolecular NMR assignments 20210609 2


Kiwifruits have become one of the most common food sources triggering allergic reactions. In patients suffering from birch pollen related food allergy, reactions result from initial sensitization to the birch (Betula verrucosa) pollen allergen Bet v 1, followed by immunological cross-reactivity to structurally homologous proteins in kiwifruit. Clinical symptoms range from scratching and itching of the oral cavity to more severe immunological reactions such as rhino conjunctivitis. In this work w  ...[more]

Similar Datasets

| S-EPMC9330593 | biostudies-literature
| S-EPMC5871579 | biostudies-literature
| S-EPMC7846771 | biostudies-literature
| S-EPMC8484069 | biostudies-literature
| S-EPMC5039217 | biostudies-literature
| S-EPMC6439177 | biostudies-literature
| S-EPMC5594049 | biostudies-literature
| S-EPMC7973641 | biostudies-literature
| S-EPMC10752501 | biostudies-literature
| S-EPMC2922568 | biostudies-literature