Strain maps characterize the symmetry of convergence and extension patterns during zebrafish gastrulation.

ABSTRACT: During gastrulation of the zebrafish embryo, the cap of blastoderm cells organizes into the axial body plan of the embryo with left-right symmetry and head-tail, dorsal-ventral polarities. Our labs have been interested in the mechanics of early development and have investigated whether these large-scale cell movements can be described as tissue-level mechanical strain by a tectonics-based approach. The first step is to image the positions of all nuclei from mid-epiboly to early segmentation by digital sheet light microscopy, organize the surface of the embryo into multi-cell spherical domains, construct velocity fields from the movements of these domains and extract strain rate maps from the change in density of the domains. During gastrulation, tensile/expansive and compressive strains in

SUBMITTER: Bhattacharya D 

PROVIDER: S-EPMC8481280 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature