Molecular principles of recruitment and dynamics of guest proteins in liquid droplets.

ABSTRACT: Despite the continuous discovery of host and guest proteins in membraneless organelles, complex host-guest interactions hinder the understanding of the molecular grammar governing liquid-liquid phase separation. In this study, we characterized the localization and dynamic properties of guest proteins in liquid droplets using single-molecule fluorescence microscopy. Eighteen guest proteins of different sizes, structures, and oligomeric states were examined in host p53 liquid droplets. Recruitment did not significantly depend on the structural properties of the guest proteins, but was moderately correlated with their length, total charge, and number of R and Y residues. In contrast, the diffusion of disordered guest proteins was comparable to that of host p53, whereas that of folded proteins

SUBMITTER: Kamagata K 

PROVIDER: S-EPMC8481498 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature