Identification of a novel B cell epitope on the nucleocapsid protein of porcine deltacoronavirus.

ABSTRACT: Porcine deltacoronavirus (PDCoV) is an emerging coronavirus that causes vomiting, diarrhea, dehydration, and even death of piglets, resulting in significant losses to the pig industry worldwide. However, the epitopes of PDCoV remain largely unknown. In this study, a monoclonal antibody (mAb) against the PDCoV nucleocapsid (N) protein, termed 9G1, was prepared using the lymphocyte hybridoma technique, and was identified as a type IgG1 with a κ light chain and reacted with the native N protein of PDCoV. Furthermore, the epitope recognized by the 9G1 mAb was subjected to western blot and an ELISA using truncated recombinant proteins and synthetic polypeptides of the PDCoV N protein. The results indicate that 9G1 mAb recognized the epitope, G⁵⁹TPIPPSYAFYY⁷⁰ (EP-9G1), a no

SUBMITTER: Wei S 

PROVIDER: S-EPMC8481650 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature