Control of Gene Expression With Quercetin-Responsive Modular Circuits.

ABSTRACT: Control of gene expression is crucial for several biotechnological applications, especially for implementing predictable and controllable genetic circuits. Such circuits are often implemented with a transcriptional regulator activated by a specific signal. These regulators should work independently of the host machinery, with low gratuitous induction or crosstalk with host components. Moreover, the signal should also be orthogonal, recognized only by the regulator with minimal interference with the host operation. In this context, transcriptional regulators activated by plant metabolites as flavonoids emerge as candidates to control gene expression in bacteria. However, engineering novel circuits requires the characterization of the genetic parts (e.g., genes, promoters, ribosome binding s

SUBMITTER: Kashiwagi FM 

PROVIDER: S-EPMC8481877 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature