The Effects of General Anaesthesia and Light on Behavioural Rhythms and GABA_A Receptor Subunit Expression in the Mouse SCN.

ABSTRACT: General anaesthesia (GA) is known to affect the circadian clock. However, the mechanisms that underlie GA-induced shifting of the clock are less well understood. Activation of γ-aminobutyric acid (GABA)_-type A receptors (GABA_AR) in the suprachiasmatic nucleus (SCN) can phase shift the clock and thus GABA and its receptors represent a putative pathway via which GA exerts its effect on the clock. Here, we investigated the concurrent effects of the inhalational anaesthetic, isoflurane, and light, on mouse behavioural locomotor rhythms and on α1, β3, and γ2 GABA_AR subunit expression in the SCN of the mouse brain. Behavioural phase shifts elicited by exposure of mice to four hours of GA (2% isoflurane) and light (400 lux) (n = 60) were determined by recording ru

SUBMITTER: Chong J 

PROVIDER: S-EPMC8482144 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature