Engineering a 3D Vascularized Adipose Tissue Construct Using a Decellularized Lung Matrix.

ABSTRACT: Critically sized defects in subcutaneous white adipose tissue result in extensive disfigurement and dysfunction and remain a reconstructive challenge for surgeons; as larger defect sizes are correlated with higher rates of complications and failure due to insufficient vascularization following implantation. Our study demonstrates, for the first time, a method to engineer perfusable, pre-vascularized, high-density adipose grafts that combine patient-derived adipose cells with a decellularized lung matrix (DLM). The lung is one of the most vascularized organs with high flow, low resistance, and a large blood-alveolar interface separated by a thin basement membrane. For our work, the large volume capacity within the alveolar compartment was repurposed for high-density adipose cell filling, wh

SUBMITTER: DeBari MK 

PROVIDER: S-EPMC8482279 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature