Multicolor fluorescence encoding of different microRNAs in lung cancer tissues at the single-molecule level.

ABSTRACT: The simultaneous detection of multiple microRNAs (miRNAs) will facilitate early clinical diagnosis. Herein, we demonstrate the integration of multicolor fluorophore-encoded cascade signal amplification with single-molecule detection for simultaneous measurement of different miRNAs in lung cancer tissues. This assay involves two linear templates and two circular templates without the requirement of any fluorescent-labeled probes. The binding of target miRNAs to their corresponding linear templates initiates the cyclic strand displacement amplification, generating many triggers which can specifically hybridize with the corresponding biotin-labeled AP probes to initiate the apurinic/apyrimidic endonuclease 1-assisted cyclic cleavage reaction for the production of more biotin-labeled primers f

SUBMITTER: Li CC 

PROVIDER: S-EPMC8482311 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature