Influence of pre-analytical sample preparation on drug concentration measurements in peritoneal tissue: an ex-vivo study.

ABSTRACT:

Objectives

Biopsy morphology (surface/depth ratio) and sample processing might affect pharmacological measurements in peritoneal tissue.

Methods

This is an ex-vivo study on inverted bovine urinary bladders (IBUB). We compared cisplatin (CIS) and doxorubicin (DOX) concentration in 81 standardized transmural punch biopsies of different diameters (6 and 12 mm). Then, we assessed the effect of dabbing the peritoneal surface before analysis. After automatized tissue homogenization with ceramic beads followed by lyophilisation, DOX concentration was quantified by high-performance liquid chromatography (HPLC), CIS concentration by atomic absorption spectroscopy. Experiments were performed in triplicate; the analysis was blinded to the sample origin. Comparisons were performe

SUBMITTER: Castagna A 

PROVIDER: S-EPMC8482446 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature