Strategies for the Site-Specific Decoration of DNA Origami Nanostructures with Functionally Intact Proteins.

ABSTRACT: DNA origami structures provide flexible scaffolds for the organization of single biomolecules with nanometer precision. While they find increasing use for a variety of biological applications, the functionalization with proteins at defined stoichiometry, high yield, and under preservation of protein function remains challenging. In this study, we applied single molecule fluorescence microscopy in combination with a cell biological functional assay to systematically evaluate different strategies for the site-specific decoration of DNA origami structures, focusing on efficiency, stoichiometry, and protein functionality. Using an activating ligand of the T-cell receptor (TCR) as the protein of interest, we found that two commonly used methodologies underperformed with regard to stoichiometry

SUBMITTER: Hellmeier J 

PROVIDER: S-EPMC8482763 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature