The use of nanobodies in a sensitive ELISA test for SARS-CoV-2 Spike 1 protein.

ABSTRACT: Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigens in the fluid has important uses in biotechnology, and is integral to many point-of-care SARS-CoV-2 diagnostics. Sandwich enzyme-linked immunosorbent assays (ELISAs) are a sensitive, well-established method of measuring antigens in solutions. They use one ligand to capture and the other ligand to detect the target analyte. Detection is commonly achieved using colorimetric readout obtained upon the reaction of a substrate with HRP-conjugated secondary ligand. Nanobodies, the V_HH domain of camelid antibodies, have expanded the repertoire of molecules used in antigen detection. Nanobodies' high affinity for target antigens, their compact structure, their high stability and ease of production has drive

SUBMITTER: Girt GC 

PROVIDER: S-EPMC8483265 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature