Epstein-Barr virus-based plasmid enables inheritable transgene expression in mouse cerebral cortex.

ABSTRACT: Continuous development of the cerebral cortex from the prenatal to postnatal period depends on neurons and glial cells, both of which are generated from neural progenitor cells (NPCs). Owing to technical limitations regarding the transfer of genes into mouse brain, the mechanisms behind the long-term development of the cerebral cortex have not been well studied. Plasmid transfection into NPCs in embryonic mouse brains by in utero electroporation (IUE) is a widely used technique aimed at expressing transgenes in NPCs and their recent progeny neurons. Because the plasmids in NPCs are attenuated with each cell division, the transgene is not expressed in their descendants, including glial cells. The present study shows that an Epstein-Barr virus-based plasmid (EB-oriP plasmid) is helpful for s

SUBMITTER: Satake T 

PROVIDER: S-EPMC8483300 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature