Comparison of methods for pre-processing, exosome isolation, and RNA extraction in unpasteurized bovine and human milk.

ABSTRACT: Milk is a highly complex, heterogeneous biological fluid that contains non-nutritive, bioactive extracellular vesicles called exosomes. Characterization of milk-derived exosomes (MDEs) is challenging due to the lack of standardized methods that are currently being used for milk pre-processing, storage, and exosome isolation. In this study, we tested: 1) three pre-processing methods to remove cream, fat, cellular debris, and casein proteins from bovine milk to determine whether pre-processing of whole milk prior to long-term storage improves MDE isolations, 2) the suitability of two standard exosome isolation methods for MDE fractionation, and 3) four extraction protocols for obtaining high quality RNA from bovine and human MDEs. MDEs were characterized via Transmission Electron Microscopy

SUBMITTER: Wijenayake S 

PROVIDER: S-EPMC8483318 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature