Biofabrication of functional protein nanoparticles through simple His-tag engineering.

ABSTRACT: We have developed a simple, robust, and fully transversal approach for the a-la-carte fabrication of functional multimeric nanoparticles with potential biomedical applications, validated here by a set of diverse and unrelated polypeptides. The proposed concept is based on the controlled coordination between Zn²⁺ ions and His residues in His-tagged proteins. This approach results in a spontaneous and reproducible protein assembly as nanoscale oligomers that keep the original functionalities of the protein building blocks. The assembly of these materials is not linked to particular polypeptide features, and it is based on an environmentally friendly and sustainable approach. The resulting nanoparticles, with dimensions ranging between 10 and 15 nm, are regular in size, are

SUBMITTER: Lopez-Laguna H 

PROVIDER: S-EPMC8483566 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature