Weakly Internalized Receptors Use Coated Vesicle Heterogeneity to Evade Competition during Endocytosis.

ABSTRACT: The uptake of receptors by clathrin-mediated endocytosis underlies signaling, nutrient import, and recycling of transmembrane proteins and lipids. In the complex, crowded environment of the plasma membrane, receptors are internalized when they bind to components of the clathrin coat, such as the major adaptor protein, AP2. Receptors with higher affinity for AP2 are known to be more strongly internalized compared to receptors with lower affinity. However, it remains unclear how receptors with different affinities compete for space within crowded endocytic structures. To address this question, we constructed receptors with varying affinities for AP2 and allowed them to compete against one another during internalization. As expected, the internalization of a receptor with high affinity for AP

SUBMITTER: DeGroot ACM 

PROVIDER: S-EPMC8483609 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature