The plant nuclear lamina proteins NMCP1 and NMCP2 form a filamentous network with lateral filament associations.

ABSTRACT: Plant genomes lack genes encoding intermediate filament proteins, including lamins; however, functional lamin analogues are presumed to exist in plants. Plant-specific coiled-coil proteins, that is, nuclear matrix constituent proteins (NMCPs), are the most likely candidates as the structural elements of the nuclear lamina because they exhibit a lamin-like domain arrangement. They are exclusively localized at the nuclear periphery and have functions that are analogous to those of lamins. However, their assembly into filamentous polymers has not yet been confirmed. In this study, we examined the higher-order structure of NMCP1 and NMCP2 in Apium graveolens cells by using stimulated emission depletion microscopy combined with immunofluorescence cell labelling. Our analyses revealed that NMCP1

SUBMITTER: Masuda K 

PROVIDER: S-EPMC8483785 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature