ZBED6 regulates Igf2 expression partially through its regulation of miR483 expression.

ABSTRACT: The expression of Igf2 in mammals shows a complex regulation involving multiple promoters and epigenetic mechanisms. We previously identified a novel regulatory mechanism based on the interaction between the transcriptional factor ZBED6 and Igf2 intron. Disruption of the ZBED6-Igf2 interaction leads to a dramatic up-regulation of IGF2 expression postnatally. In the current study we characterize an additional layer of regulation involving miR483 encoded by another Igf2 intron. We found a highly significant up-regulation of miR483 expression when the ZBED6-Igf2 axis is disrupted in transgenic mice. Furthermore, CRISPR/Cas9 mediated knock-out of miR483 in C2C12 myoblast cells, both wild-type and cells with disrupted ZBED6-Igf2 axis (Igf2^dGGCT), resulted in down-regulation of Igf2 e

SUBMITTER: Naboulsi R 

PROVIDER: S-EPMC8484269 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature