In silico characterization of the GH5-cellulase family from uncultured microorganisms: physicochemical and structural studies.

ABSTRACT:

Background

Hydrolysis of cellulose-based biomass by cellulases produce fermented sugar for making biofuels, such as bioethanol. Cellulases hydrolyze the β-1,4-glycosidic linkage of cellulose and can be obtained from cultured and uncultured microorganisms. Uncultured microorganisms are a source for exploring novel cellulase genes through the metagenomic approach. Metagenomics concerns the extraction, cloning, and analysis of the entire genetic complement of a habitat without cultivating microbes. The glycoside hydrolase 5 family (GH5) is a cellulase family, as the largest group of glycoside hydrolases. Numerous variants of GH5-cellulase family have been identified through the metagenomic approach, including CelGH5 in this study. University-CoE-Research Center for Biomolecule Enginee

SUBMITTER: Sanjaya RE 

PROVIDER: S-EPMC8484414 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature