Functional and histologic imaging of urinary bladder wall after exposure to psychological stress and protamine sulfate.

ABSTRACT: To quantify the urinary bladder wall T₁ relaxation time (T₁) before and after the instillation contrast mixture in rats previously subjected to water avoidance stress (WAS) and/or acute exposure to protamine sulfate (PS). Female Wistar rats were randomized to receive either sham (control) or 1 h of WAS for ten consecutive days before the evaluation of nocturnal urination pattern in metabolic cages. T₁ mapping of urinary bladder wall at 9.4 T was performed pre- and post- instillation of 4 mM Gadobutrol in a mixture with 5 mM Ferumoxytol. Subsequently, either T₁ mapping was repeated after brief intravesical PS exposure or the animals were sacrificed for histology and analyzing the mucosal levels of mRNA. Compared to the control group, WAS exposure

SUBMITTER: Saito T 

PROVIDER: S-EPMC8484474 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature