Growth and site-specific organization of micron-scale biomolecular devices on living mammalian cells.

ABSTRACT: Mesoscale molecular assemblies on the cell surface, such as cilia and filopodia, integrate information, control transport and amplify signals. Designer cell-surface assemblies could control these cellular functions. Such assemblies could be constructed from synthetic components ex vivo, making it possible to form such structures using modern nanoscale self-assembly and fabrication techniques, and then oriented on the cell surface. Here we integrate synthetic devices, micron-scale DNA nanotubes, with mammalian cells by anchoring them by their ends to specific cell surface receptors. These filaments can measure shear stresses between 0-2 dyn/cm², a regime important for cell signaling. Nanotubes can also grow while anchored to cells, thus acting as dynamic cell components. This app

SUBMITTER: Jia S 

PROVIDER: S-EPMC8484582 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature