Identification of Potential Biomarkers for Liver Cancer Through Gene Mutation and Clinical Characteristics.

ABSTRACT: Liver cancer is a common malignant tumor worldwide, which is a serious threat to the health of people. We try to investigate some mutations and clinical indicators as candidate markers for the development of liver cancer through targeted region capture technology combined with next-generation sequencing. We collected peripheral blood and liver cancer tissue samples from 32 liver patients concurrently. The SeqCap EZ Prime Choice Probe was used to perform the targeted enrichment; this probe captures 1,000 known cancer-associated genes. We calculated the tumor mutation burden (TMB) for each patient. The high-frequency mutations and these relative genes were identified. Eventually, survival analysis was performed based on the mutations and clinical indicators. In 32 liver patients, a total of

SUBMITTER: Cui Y 

PROVIDER: S-EPMC8484954 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature