Pre-clinical evaluation of immunoPET imaging using agonist CD40 monoclonal antibody in pancreatic tumor-bearing mice.

ABSTRACT:

Background

A novel [⁶⁴Cu]Cu-NOTA-aCD40 immunoPET tracer was developed to image a CD40⁺ pancreatic tumor model in C57BL/6 mice and to study the biodistribution profile of the agonist CD40 (aCD40) monoclonal antibody (mAb) alone or combined with other mAbs.

Procedures

Copper-64 ([⁶⁴Cu]Cu) labeled NOTA-aCD40 and NOTA-IgG (10 μg; 7 MBq) were injected intravenously into C57BL/6 mice with subcutaneous mT4 tumors to assess specificity 48 h post injection (p.i.) through positron emission tomography/computed tomography (PET/CT) imaging and biodistribution studies (n = 5). [⁶⁴Cu]Cu-NOTA-aCD40 was injected alone or simultaneously in combination with a therapeutic mass of cold aCD40 (100 μg), aPD-1 (200 μg) and aCTLA-4 (200 μg) mAbs. A group o

SUBMITTER: Aghevlian S 

PROVIDER: S-EPMC8486004 | biostudies-literature | 2021 Jul-Aug

REPOSITORIES: biostudies-literature