Advances in Agrobacterium transformation and vector design result in high-frequency targeted gene insertion in maize.

ABSTRACT: CRISPR-Cas is a powerful DNA double-strand break technology with wide-ranging applications in plant genome modification. However, the efficiency of genome editing depends on various factors including plant genetic transformation processes and types of modifications desired. Agrobacterium infection is the preferred method of transformation and delivery of editing components into the plant cell. While this method has been successfully used to generate gene knockouts in multiple crops, precise nucleotide replacement and especially gene insertion into a pre-defined genomic location remain highly challenging. Here, we report an efficient, selectable marker-free site-specific gene insertion in maize using Agrobacterium infection. Advancements in maize transformation and new vector design enabled

SUBMITTER: Peterson D 

PROVIDER: S-EPMC8486252 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature