Analysis of the mechanism of damage produced by thiazole orange photoinactivation in apheresis platelets.

ABSTRACT:

Background

Pathogen Reduction Technologies (PRTs) are broad spectrum nucleic acid replication-blocking antimicrobial treatments designed to mitigate risk of infection from blood product transfusions. Thiazole Orange (TO), a photosensitizing nucleic acid dye, was previously shown to photoinactivate several types of bacterial and viral pathogens in RBC suspensions without adverse effects on function. In this report we extended TO treatment to platelet concentrates (PCs) to see whether it is compatible with in vitro platelet functions also, and thus, could serve as a candidate technology for further evaluation.

Material and methods

PCs were treated with TO, and an effective treatment dose for inactivation of Staphylococci was identified. Platelet function and physiology were th

SUBMITTER: Gough P 

PROVIDER: S-EPMC8486609 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature