Overexpression of lncRNAs with endogenous lengths and functions using a lncRNA delivery system based on transposon.

ABSTRACT:

Background

Long noncoding RNAs (lncRNAs) play important roles in many physiological and pathological processes, this indicates that lncRNAs can serve as potential targets for gene therapy. Stable expression is a fundamental technology in the study of lncRNAs. The lentivirus is one of the most widely used delivery systems for stable expression. However, it was initially designed for mRNAs, and the applicability of lentiviral vectors for lncRNAs is largely unknown.

Results

We found that the lentiviral vector produces lncRNAs with improper termination, appending an extra fragment of ~ 2 kb to the 3'-end. Consequently, the secondary structures were changed, the RNA-protein interactions were blocked, and the functions were impaired in certain lncRNAs, which indicated that lentivi

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC8487477 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature