Evaluation of candidate reference genes for quantitative real-time PCR analysis in a male rat model of dietary iron deficiency.

ABSTRACT:

Background

Quantitative real-time polymerase chain reaction (qPCR) is a reliable and efficient method for quantitation of gene expression. Due to the increased use of qPCR in examining nutrient-gene interactions, it is important to examine, develop, and utilize standardized approaches for data analyses and interpretation. A common method used to normalize expression data involves the use of reference genes (RG) to determine relative mRNA abundance. When calculating the relative abundance, the selection of RG can influence experimental results and has the potential to skew data interpretation. Although common RG may be used for normalization, often little consideration is given to the suitability of RG selection for an experimental condition or between various tissue or cell types.

SUBMITTER: Fiddler JL 

PROVIDER: S-EPMC8487497 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature