Distinct B cell subsets in Peyer's patches convey probiotic effects by Limosilactobacillus reuteri.

ABSTRACT:

Background

Intestinal Peyer's patches (PPs) form unique niches for bacteria-immune cell interactions that direct host immunity and shape the microbiome. Here we investigate how peroral administration of probiotic bacterium Limosilactobacillus reuteri R2LC affects B lymphocytes and IgA induction in the PPs, as well as the downstream consequences on intestinal microbiota and susceptibility to inflammation.

Results

The B cells of PPs were separated by size to circumvent activation-dependent cell identification biases due to dynamic expression of markers, which resulted in two phenotypically, transcriptionally, and spatially distinct subsets: small IgD⁺/GL7^-/S1PR1⁺/Bcl6, CCR6-expressing pre-germinal center (GC)-like B cells with innate-like funct

SUBMITTER: Liu HY 

PROVIDER: S-EPMC8487498 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature